Second observation of S. niagarae, this time to show the presence of chitin fibrils, just like its close relative in the Stephanodiscaceae, Cyclotella (or Stephanocyclus). The second shot shows a full fibril. It is twice as long as the cell is wide. I can't find literature reports of fibrils on this genus, but maybe the whole family has them.
Net plankton sample. Floating at the surface via aerotopes.
GomphosphaeriasalinaKomárek& Hindák1988
Cyanobacteria
Site 2 –Port Bougainville
Bioblitz
Pennekamp State Park, DagnyHammock South section Port Bougainvillea
Key Largo, Florida
April 6 2024
Microalgae and some macroalgalphotomicrographs
Site 1 –borrow pit; phytoplankton and benthic sample
25.1783°N, 80.3659°W
Site 2 –Port Bougainville, connection to Hawk Channel; phytoplankton and benthic sample
25.1735°N, 80.3655°W
Site 3 –Shallow pond at landward edge of mangroves
25.1812°N, 80.33572°W
Tom Frankovich, Anna Nesterovich, Katherine Johnson , Mike Sullivan and other students
Chroococcuscf. pulcherrimusWelsh 1965
Site 3
Bioblitz
Pennekamp State Park, DagnyHammock South section Port Bougainvillea
Key Largo, Florida
April 6 2024
Microalgae and some macroalgalphotomicrographs
Site 1 –borrow pit; phytoplankton and benthic sample
25.1783°N, 80.3659°W
Site 2 –Port Bougainville, connection to Hawk Channel; phytoplankton and benthic sample
25.1735°N, 80.3655°W
Site 3 –Shallow pond at landward edge of mangroves
25.1812°N, 80.33572°W
Tom Frankovich, Anna Nesterovich, Katherine Johnson , Mike Sullivan and other students
Johannesbaptistia floridana Berthold and Laughinghouse 2020
Cyanobacteria
Site 2 –Port Bougainville
Bioblitz
Pennekamp State Park, DagnyHammock South section Port Bougainvillea
Key Largo, Florida
April 6 2024
Microalgae and some macroalgalphotomicrographs
Site 1 –borrow pit; phytoplankton and benthic sample
25.1783°N, 80.3659°W
Site 2 –Port Bougainville, connection to Hawk Channel; phytoplankton and benthic sample
25.1735°N, 80.3655°W
Site 3 –Shallow pond at landward edge of mangroves
25.1812°N, 80.33572°W
Tom Frankovich, Anna Nesterovich, Katherine Johnson , Mike Sullivan and other students
Johannesbaptistia floridana Berthold and Laughinghouse 2020
Cyanobacteria
Site 2 –Port Bougainville
Bioblitz
Pennekamp State Park, DagnyHammock South section Port Bougainvillea
Key Largo, Florida
April 6 2024
Microalgae and some macroalgalphotomicrographs
Site 1 –borrow pit; phytoplankton and benthic sample
25.1783°N, 80.3659°W
Site 2 –Port Bougainville, connection to Hawk Channel; phytoplankton and benthic sample
25.1735°N, 80.3655°W
Site 3 –Shallow pond at landward edge of mangroves
25.1812°N, 80.33572°W
Tom Frankovich, Anna Nesterovich, Katherine Johnson , Mike Sullivan and other students
From a plankton net sample at the end of the pier, to 7 m depth. The leftmost 'filament' is 76 x 16 µm. I'm at a total loss here. The plankton net scraped the pier pilings occasionally - maybe it's an immature macrophyte? Red alga? I think those are pyrenoids, so chlorophyte? @roman_romanov, any ideas?
Kains Lake near tower, Port Hardy, Vancouver Island, BC, Canada
Surface water grab from the Matchedash Bay - South Beaver Pond.
Habitat: shallow water along lakeshore.
From a plankton sample from the end of the pier. Bicosoeca was abundant growing epiphytically on all or most of the diatoms in the sample. In this case, they are attached to Stephanodiscus niagarae. At high magnification, the two flagella were apparent. One is either extended out the opening of the 'calyx' or or coiled around the front of the cell. The other is usually attached to the bottom of the calyx, and is retractile, allowing the cell to rapidly retreat to the bottom of the calyx (I'm using the vocabulary of James-Clark - see ref. below). When the cell retracts (possible for small cells), the entire front of the calyx collapses and resembles a rod attached to the cell. This extraordinary action is visible in a video I've put on fb, here: https://www.facebook.com/100070304167055/videos/795501582250207. The calyx is collapsed on the top two cells in that video, but you can see it slowly beginning to open by end of the shot. Short videos of the other cells on the same diatom are posted as comments.
I would not have been able to interpret these results without the help of a reference to the original publication of James-Smith, provided by @roman_romanov (here: https://www.biodiversitylibrary.org/item/32326#page/507/mode/1up) in a comment to a post by @closterium_mysterium, here: https://inaturalist.ca/observations/189118870. Thanks to both for sharing!